- Ultra-Violet Visible Spectroscopy: Ultraviolet-visible (UV-Vis) spectroscopy is an analytical technique that measures the absorption or reflection of ultraviolet and visible light by a substance. It’s primarily used to quantify the amount of a substance (analyte) in a solution by relating the amount of light absorbed to the analyte concentration. The technique is based on the principle that molecules absorb specific wavelengths of light in the UV and visible range, causing electronic transitions within the molecule. Principle: When a molecule absorbs UV or visible light, its electrons jump to a higher energy level. The amount of light absorbed at a specific wavelength is related to the concentration of the substance. Common uses: Some common applications include measuring the concentration of proteins, nucleic acids, and other biomolecules, determining the purity of compounds, and monitoring chemical reactions.
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